Composite

Part:BBa_K510031:Design

Designed by: Paola Gallardo, Rafael R. Daga   Group: iGEM11_UPO-Sevilla   (2011-09-20)

GFP-Tadh1- tetO4- Tact1


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 1262
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1262
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1262
    Illegal BamHI site found at 627
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 1262
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 1262
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Source

Obtained by DNA synthesis process.

References

Kelemen JZ, Ratna P, Scherrer S, Becskei A (2010) Spatial Epigenetic Control of Mono- and Bistable Gene Expression. PLoS Biol 8(3): e1000332. doi:10.1371/journal.pbio.1000332


Ratna P, Scherrer S, Fleischli C, Becskei A (2009) Synergy of Repression and Silencing Gradients Along the Chromosome. J. Mol. Biol. 387, 826–839 doi:10.1016/j.jmb.2009.02.025